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Fig. 2. Cleft lip and palate in Nestin cre;Bmpr1a n/f mutant embryos. (A-D) Frontal (A,B) and lateral (C,D) views of 16.5 dpc wild-type (A,C) and (B,D) Nestin cre;Bmpr1a n/f mutants showing the bilateral cleft lip in mutant embryos (arrow). Star in A and C indicates area of lip fusion in wild-type embryos. (E-J) Hematoxylin and Eosin staining of coronal sections through the palatal region of wild-type (E,G,I) and Nestin cre;Bmpr1a n/f mutant embryos (F,H,J) at the labeled developmental stages. Arrows denote unfused palatal shelves. (K-N) Coronal sections stained with Hematoxylin and Eosin of wild-type (K,M) and Nestin cre;Bmpr1a n/f mutant (L,N) secondary palate explants cultured overnight. Palatal shelves were harvested at 14.5 dpc (K,L) and E13.5 (M,N). Arrows denote the degenerating medial edge epithelium (MEE) in K and L. ns, nasal septum; ps, palatal shelf; t, tongue.