Fig. 1. Ovol1 RNA expression in adult and prepubertal testis. (A-F) In situ hybridization of testis from 8-week-old (A-D) or 16-day-old (E,F) mice using a Ovol1 cRNA probe (A,C,E), or counterstained with an -Ldhc4 antibody (B,D,F). The following stages were identified according to cell sizes, positions within the tubule and relative intensities of -Ldhc4 staining (Hintz and Goldberg, 1977): p, pachytene spermatocytes; r, round spermatids; e, elongate spermatids. No hybridization signal was observed using a sense probe (not shown). (G) Northern blot analysis detecting multiple Ovol1 transcripts in testis during prepubertal development. Numbers at the top of each lane indicate the postnatal ages at which testis samples were taken. The same blots were then stripped and hybridized with a Gapdh probe. Scale bar: 25 µm in A-D; 30 µm in E-F.