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Fig. 3. Accumulation of Gcna1+ germ cells and reduction of Ldhc4+/histone H1t+ germ cells in testis of juvenile Ovol1-/- mice. (A-D) Immunohistochemical detection of the Gcna1 protein in testis from Ovol1+/+ (A,C) and Ovol1-/- (B,D) mice at P14 and P16 using an {alpha}-Gcna1 antibody. (E-L) Immunofluorescence staining of testis from Ovol1+/+ (E,G,I,K) and Ovol1-/- (F,H,J,L) mice at P14 and P16 using an {alpha}-Ldhc4 antibody (E-H) and an {alpha}-histone H1t antibody (I,J). (K,L) Merged images between G and I, and H and J, respectively. Scale bar: 55 µm in A-F; 60 µm in G-L.