Fig. 4. Expression and localization of cyclin B1 protein in wild-type (A,C,D,F,G,I)
and Ovol1-/- (B,E,H) testis. Shown are results of
immunostaining experiments using
-cyclin B1 (A-C) and
-Ldhc4
(D-F) antibodies on P16 (A,B,D,E,G,H) and P21 (C,F,I) testis. (G-I) Merged
images. Asterisk in G-I indicates mitotic spermatogonia at the tubule
periphery that stained positive for cyclin B1. Arrows and arrowheads indicate
cytoplasmic and nuclear cyclin B1 in primary spermatocytes, respectively.
Scale bar: 25 µm.