Fig. 3. Comparison of the MYB23 and GL1 expression during leaf development. (A) RT-PCR using MYB23 gene-specific primers (35 cycles, top row) or using GL1 gene-specific primers (35 cycles, middle row). RT-PCR using primers for translation elongation factor EF1A4 (20 cycles) were used as a cDNA control. The gl1-1 allele has a deletion of the entire protein-coding region, resulting in the absence of GL1 mRNA (Oppenheimer et al., 1991). (B) Comparison of MYB23::GUS and GL1::GUS expression (blue) in leaves of wild type (WT), ttg1 and gl1 mutants.