Fig. 9. Localization of nectin 1 and nectin 3 at the puncta adherentia junctions between the pigment and non-pigment epithelia of the ciliary body. (A,B) Localization of nectin 1 (A) and nectin 3 (B) at the ciliary body of the adult wild-type eye in immunoelectron microscopy. PE, pigment epithelium; NPE, non-pigment epithelium; M, melanin; N, nucleus. Arrows indicate immunogold particles; arrowhead indicates a gap junction. Scale bars: 500 nm. (C,D) The expression patterns of nectin 1 (C) and nectin 3 (D) mRNAs at the ciliary body of the adult wild-type eye were analyzed by in situ hybridization. Nectin 1 (C, part a) and nectin 3 (D, part a) mRNA. (c) Sense controls for the nectin 1 and nectin 3 mRNAs. (b,d) Corresponding DIC images. Insets show higher magnifications of the boxed areas. Scale bars: 50 µm.