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Fig. 4. Pelvic abnormalities in Alx4 mutants. (A-E) Skeletal staining of pelvic girdles of E16.5 embryos. Black arrowhead indicates a reduction or absence of the pubic bone in Alx4 (B), Alx3/Alx4 (C), Alx4/Cart1 (D) mutants, whereas the pelvic girdle in Tbx15 mutants (E) was not affected.