Fig. 7. Decreased expression of Hoxc6 and Hoxc8 in Raldh2^L–/– brachial LMC. (A-D) Combined Raldh2 immunofluorescence (green, left side) and Hoxc6 (A,B, red) or Hoxc8 (C,D, red) in-situ hybridization on dissected spinal cords. In Raldh2^L–/– mutant, Hoxc6 expression is diminished preferentially within its central domain (A,B, brackets). (E,F) Combined Hoxc8 immunofluorescence (green) and Lim1 in-situ hybridization (red). A posterior shift of Hoxc8 protein is observed in Raldh2^L–/– spinal cord, mostly within the Lim1+ motor column (red arrows). (G-L) Hoxc8 transcripts on serial transverse sections at levels indicated in C and D; 70 µm separate each section plane. The inset in L shows the following posterior section in which Hoxc8 transcript distribution in the mutant is comparable to the previous control section (compare with K). Two red arrows delineate the Lim1+ LMC domain. a, anterior; p, posterior.