Fig. 3. KIS-L binds a large number of sites on polytene chromosomes. (A) KIS-L (green) binds to 300 sites on wild-type polytene chromosomes and is not associated with the heterochromatic chromocenter (white arrowhead). (B) Higher magnification of the distal region of chromosome arm 2R. KIS-L (green) is compared with DNA (blue); the last two panels show the merged and split images of the first two panels. KIS-L is primarily associated with interband regions.