Fig. 8. The levels of KIS-L are dramatically reduced on polytene chromosomes of kis mutant larvae. KIS-L (red; C and D) was detected on chromosomes of wild type (A,C) and kis^k13416 (B,D) larvae. DNA (blue; A,B) was visualized by DAPI staining. The level of KIS-L associated with chromatin is drastically reduced in kis mutants, but the overall banding pattern and morphology of polytene chromosomes is normal.