(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. RT-PCR expression analysis of XEN cells. cDNA was prepared from ES cells, embryoid bodies (EB) at day 5 (D5) and day 7 (D7), and three different XEN cell cultures. XEN cells were either cultured on gelatin in maintenance conditions (XEN) or differentiated (Diff) by removal of EMFI-CM and gelatin for 4 or 8 days (D4, D8). Hnf4 and Foxa2 were consistently detected in all XEN cell cultures, while Gata4 and Sox7 decreased expression upon differentiation. By contrast, {alpha}-fetoprotein (Afp) increased with differentiation. Oct4 was undetectable in XEN cells.