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Fig. 4. XEN cell chimeras. (A,B) E6.5 chimera with XEN cells contributions to the distal region of the conceptus in the parietal yolk sac: (A) Partial phase-contrast and UV fluorescence micrograph; (B) UV fluorescence micrograph with false-color added. (C,D) Two E7.5 chimeras with XEN cell contributions exclusively to the parietal yolk sac; phase-contrast (C) and UV fluorescence micrographs (D). (E) E8.5 XEN cell chimera in which the parietal yolk sac was dissected from the visceral yolk sac and embryo proper after X-gal staining. All the X-gal-positive cells were located in the parietal yolk sac and not in other tissues. (F) E7.5 XEN cell chimera after X-gal staining. A coherent XEN cell clone was observed in the extra-embryonic region of the visceral endoderm.