Fig. 6. PTEN is required for organization of the actin cytoskeleton during oogenesis. (A) Eggs derived from Pten germ-line clones are generally smaller than wild-type eggs. The number of eggs counted for each genotype was plotted against egg size (in µm). Fifty eggs were counted for each genotype. (B) Wild-type eggs are long and slender, whereas eggs derived from Pten germ-line clones (C,D) are shorter and more rounded. (E) In a wild-type egg chamber at stage 10, actin (E') is localized along the cell borders of the nurse cells, in ring canals (arrowheads) and underlying the plasma membrane of the oocyte. The Staufen protein (E'') is localized to the anterior and posterior pole of the oocyte. (F) In an egg chamber at stage 10 in which the germline is mutant for Pten, actin (F') is disorganized but the localization of Staufen (F'') is normal. (G) In a wild-type egg chamber at stage 10, the nurse cell nuclei (G', visualized with DAPI) do not penetrate into the oocyte. (H) By contrast, in many stage 10 egg chambers with Pten germ-line clones, nurse cell nuclei are found within the oocyte. Clones of homozygous Pten mutant cells were marked by absence of GFP fluorescence. The weak green fluorescence signal in H is bleed through from the follicle cells, as images in G and H were taken with a conventional epifluorescence microscope in order to visualize DAPI. The oocyte is circled with a broken white line in G and H. In all panels, anterior is towards the left. nc, nurse cell; oo, oocyte. Scale bar: 100 µm.