Fig. 2. Effects of NPA and BFA on vein patterning. Vein patterns of first-node leaves of the wild-type (A-D,I-L) and the van3 mutant (E-H,M-P) grown for 11 days on agar medium containing NPA (A-H) or BFA (I-P). Mock-treated leaves are shown in A,E,I,M. Chemicals were applied at concentrations of 5 µM (B,F,J,N), 10 µM (C,G,K,O), or 20 µM (D,H,L,P). Inset in L is a close-up of a trachery element island under Nomarski optics. Scale bars: (A-P), 500 µm.