Fig. 6. Subcellular localization of VAN3. (A) Arabidopsis suspension-cultured cells. (B-D,G-I) Location of VAN3–Venus (red) and GFP-tagged subcellular marker genes (green). 35S-promoter-driven Venus-tagged VAN3 and GFP-tagged subcellular markers were co-introduced into protoplasts of Arabidopsis suspension-cultured cells. (B-D) Merged image of VAN3–Venus and the ER marker HDEL–GFP (B), the cis-Golgi marker SYP31–GFP (C), the endosome marker ARA6–GFP (D). (E) Location of ARA7–GFP in the gnom mutant cells. (F) VAN3–Venus in gnom mutant cells. (G–I) Localization of the TGN marker SYP41–GFP (G) and VAN3–Venus (H), and a merged image of G and H (I). E and F are projection images of serial confocal planes, A-D and G-I and single confocal slice images. Scale bars: (A-I) 5 µm.