Fig. 5. BAY 11-7082 reduces neurite outgrowth from nodose neurons. P0 nodose neurons were incubated in medium containing 10 ng/ml BDNF and either the IB- phosphorylation inhibitor BAY 11-7082 at a concentration of 10 µmol/l or vehicle control. Vehicle-treated neurons were also grown without BDNF. (A) Percent survival after 48 hours incubation. (B) Total neurite length after 24 hours incubation. (C) Number of branching points in neurite arbors after 24 hours incubation. (D) Sholl analysis of neurite arbor morphology after 24 hours incubation. (E) Photomicrograph of a typical ßIII tubulin stained, vehicle-treated neuron grown for 24 hours with BDNF. (F) Photomicrograph of a typical ßIII tubulin stained, BAY 11-7082 treated neuron grown for 24 hours with BDNF. The means and standard errors of data obtained from at least 40 neurons in each experimental condition shown. Statistical comparisons shown are with respect to the control transfected neurons, *P<0.05, **P<0.001. Scale bars: 50 µm.