(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. {kappa}B decoy DNA reduces neurite growth from nodose neurons. P0 nodose neurons were transfected with a YFP expression plasmid together with either a {kappa}B decoy DNA or a scrambled control variant and were incubated in medium containing 10 ng/ml BDNF. Control transfected neurons were also grown without BDNF. (A) Percent survival 48 hours after transfection. (B) Total neurite length 24 hours after transfection. (C) Number of branching points in neurite arbors 24 hours after transfection. (D) Sholl analysis of neurite arbor morphology 24 hours after transfection. (E) Photomicrograph of a typical neuron transfected with the scrambled control oligonucleotide and grown for 24 hours with BDNF. (F) Photomicrograph of a typical neuron transfected with the {kappa}B decoy oligonucleotide and grown for 24 hours with BDNF. Statistical comparisons shown are with respect to the control transfected neurons, *P<0.05, **P<0.001. Scale bars: 50 µm.