Fig. 1. Genomic structures of PpLFY1 and PpLFY2 in the wild type and the targeted lines. The gene structures of the wild-type PpLFY1 (A) and PpLFY2 (B). Restriction enzyme recognition sites are indicated on each gene: b, BglII; e, EcoRI. In A,B,D-G the boxes indicate exons and the lines between the boxes, introns; circles, putative start codons and squares, putative stop codons. The DNA fragments that were used as probes in the Southern analyses are indicated with thick lines below each gene in A and B. (C) Southern analyses of PpLFY1 and PpLFY2 in the wild type. The restriction enzymes, probes, and stringency used are indicated above or below each image. (D-G) Genomic structures of PpLFY1 (D,F) and PpLFY2 (E,G) in PpLFY1-GUS lines (D), PpLFY2-GUS lines (E), PpLFY1-dis and PpLFY1-PpLFY2-dis lines (F), PpLFY2-dis and PpLFY1-PpLFY2-dis lines (G). uidA, uidA coding region; nos-ter, nopaline synthase polyadenylation signal; NPTII, NPTII expression cassette; HPT, HPT expression cassette. The sense directions of uidA, nptII, and hpt are indicated with arrows. The scale bar applies to A,B,D-G.