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Fig. 4. The phenotypes of the single and double disruptants of the PpLFY1 and PpLFY2 genes. (A) A sporophyte (arrow) is formed on the shoot apex of a gametophore. (B-E) Gametophores of wild-type P. patens (B), PpLFY1-dis-1 (C), PpLFY2-dis-1 (D), and PpLFY1-PpLFY2-dis-2 (E) lines, photographed from above. The sporangia are ocher to dark brown spheres, two of which are indicated in each panel by arrowheads. Scale bars: 1 mm.