Fig. 10. Loss-of-function effects of RA, Hh and FGFR signaling pathways on eye DV polarity. Embryos were treated from stage 10.5 with 10 µM AGN194310 (AGN), 100 µM cyclopamine (CPM) and 25 µM SU5402 (SU) in different combinations, and analyzed for molecular marker expression at stage 30/31. (A) Effects of the single inhibition of any of the RA, Hh and FGFR signaling pathways, when compared with mock-treated embryos. (B) Effects of double and triple inhibition of RA, Hh and FGFR pathways. (C) Schematic representation of the results shown in A and B. Strong eye dorsalization is caused by triple inhibition of RA, Hh and FGFR signaling, while double inhibitions produce weaker effects.