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Fig. 4. Target validation. Embryonic expression of pal-1, assayed by YFP reporter, is dependent on maternal PAL-1 activity. (A-C) Expression of a zygotic pal-1 reporter is shown in a mid-gastrula embryo with corresponding Nomarski images for wild-type (A,D), pal-1(RNAi) (B,E) and mex-3(RNAi) (C,F).