Fig. 8. Projection of three-dimensional images of reporter expression patterns for PAL-1 target genes likely to be involved in patterning. Dorsal and lateral views of chromosomally integrated transcriptional reporters 210 minutes after the four-cell stage (22°C; 200 cells, 16 C-lineage cells) are shown for 13 validated targets. Images are orthogonal perspectives following three-dimensional volume rendering of many optical sections. A broken gray line marks the approximate outline of each egg case. (A) Ordination: 8 C ectodermal nuclei in blue, 8 C muscle nuclei in red and 4 D muscle nuclei in yellow; (B) pal-1; (C) vab-7; (D) cwn-1; (E) C55C2.1; (F) unc-120; (G) hnd-1; (H) hlh-1; (I) elt-3; (J) mab-21; (K) nhr-25; (L) nob-1; (M) tbx-8; (N) tbx-9. Movies for each reporter are available in the supplementary material.