Fig. 1. DjPum encodes a homologue of Drosophila Pumilio. (A) Nucleotide sequence and predicted protein product of the planarian DjPum cDNA. Grey background indicates the highly conserved PUF repeat domain and the flanking regions. DjPum Accession Number is AJ639658. (B) Comparison of PUF repeats and flanking regions of DjPum (Dj) with the corresponding regions of Drosophila Pumilio (Dm) and human Pumilio 2 (Hs2). Amino acid sequences are aligned using CLUSTAL W and visualized with BOXSHADE. Black backgrounds indicate identical residues and grey backgrounds indicate conserved residues. (C) Phylogenetic tree based on the PUF repeats and the flanking regions of PUF proteins of different species. DjPum (DJ) is circled. Accession numbers: Caenorhabditis elegans Ce1 (FBF-1), AAF39879 Ce2 (FBF-2), Q09312; Ce3 (PUF-3), CAB63369 Ce4 (PUF-4), T33752; Ce5 (PUF-5), T22634; Ce6 (PUF-6), T21080; Ce7 (PUF-7), T32528; Ce8 (PUF-8), T15717; Ce9 (PUF-9), T26218; Ce10 (PUF-10), AAF60691 Ce11 (PUF-11), AAK68592 Drosophila melanogaster Dm (Pumilio), A46221; Leishmania major Lm, CAB62815 Trypanosoma brucei Tb, AAK62674 Saccharomyces cerevisiae Sc1 (JSN1), P47135; Sc2 (PUF2), NP-015367; Sc3 (PUF3), NP-013088; Sc4 (PUF4), P25339; Sc5 (PUF5/MPT5/UTH4), P39016; Sc6 (PUF6), S69554; Schizosaccharomyces pombe Sp1, CAB60694 Sp2, Q09829; Sp3, Q92359; Sp4, Q10238; Sp5, CAA20674 Sp6, CAA18887 Sp7, CAB54870 Neurospora crassa Nc, T49434; Dictyostelium discoideum Dd (PufA), AAD39751 Homo sapiens Hs1, AAG31807 Hs2, AAG31806 Mus musculus Mm1, AAG42319 Mm2, AAG31805 Xenopus laevis Xl, BAB20864 Arabidopsis thaliana At1, AAC95220 At2, AAC95216 At3, AAF02808 At4, AL049480; At5, BAA97177 At6, AAF87849 At7, CAB82120 At8, AAC28191 At9, AC007727; Oryza sativa Os, AAK73144 Populus tremula x Populus tremuloides Pt, AAF71823