Fig. 7. Characterization of the cell fraction obtained following the serial filtering procedure in D. japonica. (A) Bright-field image of cells obtained by filtration through the 8 µm pore size mesh. Scale bar: 10 µm. (B) DjMCM2 and DjFGFR1 expression visualized by comparative RT-PCR. RNA was extracted from cell fractions obtained by filtration through nylon meshes of 50 µm (1), 20 µm (2) and 8 µm (3) pore size sequentially. DjEF2 is used as an internal amplification control.