Fig. 4. Expression pattern of alr-1. (A-D) Embryonic expression of ALR-1. (A) Nomarski image and (B) anti-ALR-1 antibody staining of a 1.5-fold embryo. ALR-1 expression is observed in two cells in the anterior, only one of which is visible in this plane of focus. (C) Nomarski and (D) anti-ALR-1 antibody stained threefold embryo exhibiting ALR-1 expression in multiple cells in the head, body and tail. Shown is a merge of three different focal planes. (E-J) Post-embryonic expression of ALR-1. Adult animals expressing the GABAergic neuronal marker unc-47::gfp in the head (E,F) or tail (G,H) were co-stained with anti-ALR-1 antibodies. Identified cells co-expressing both markers are labeled. Ventral cord motoneurons expressing both markers are indicated by arrowheads. Additional ALR-1-expressing cells were not identified. (I,J) Adult animals stained with anti-ODR-7 antibodies (I) or expressing ops-1::gfp (J) were stained with anti-ALR-1 antibodies. ODR-7-expressing AWA neurons and ops-1::gfp-expressing ASG neurons are indicated by arrows. No ALR-1 expression was observed in these neurons. Scale bar: 10 µm.