Fig. 7. Shh-independent oligodendrocyte development from Dbx-derived precursors. Dissociated spinal cords from Dbx1-iCrexRosa26-GFP embryos developed Sox10-positive OLPs within 5 days of culture (A). Their development was not inhibited by cyclopamine (B), but was abolished in the presence of PD173074 (C) or both (D). (E) The experiment was quantified by counting the total number of double-labelled GFP-positive/Sox10-positive cells in the cultures. A minimum of 1000 Sox10-positive cells was counted for each condition. Similar data were obtained in two independent experiments.