Fig. 8. Fibrous and protoplasmic astrocytes are derived from the Dbx-expressing neuroepithelium. (A) Co-localization between GFAP (red) and GFP (green) in fibrous astrocytes in the developing white matter of a P10 spinal cord. (B) Confocal microscope view of the area highlighted in A. (C-F) Protoplasmic astrocytes develop from Dbx precursors. (C) All GFP-positive protoplasmic astrocytes co-label with anti-S100ß antibody (red). (D) Protoplasmic astrocytes next to the central canal weakly co-expressed GFAP. (E) GFP and NeuN (red) double labelling in the ventral horn, lying in close proximity to motoneuron cell bodies. (F) Protoplasmic-like astrocytes form bilateral longitudinal columns of cells positioned adjacent to the central canal (arrows). Nuclei are stained with DAPI (blue). Scale bars: in A, 200 µm; in C, 20 µm.