Fig. 2. Expression of Dcc in early-born dorsal interneurons. (A) Immunocytochemical staining of Dcc of E11.5 neural tube shows Dcc expression in the region lining the VZ of the neural tube. (B) Double immunocytochemical staining of Dcc (green) and Isl1 (red) in E11.5 dorsal spinal cord shows Isl1 in the nuclei and Dcc on membrane and in axons. (C) Higher magnification of B. Some Isl1⁺ cells were not stained for Dcc (arrow in C). (D) Double staining of Dcc (green) and Lmx1b (red). (E) Higher magnification of (D). Some Dcc⁺ cells do not express Lmx1b (arrowhead in E). (F) Double staining of Dcc (green) and Pax2 (red; dI4 and dI6 marker). (G) Higher magnification of dI4 region. (H) Higher magnification of dI6 region. Double arrowheads indicate double labeled-neurons. Scale bars: 100 µm in A; 50 µm in B,D,F; 25 µm C,E,G,H.