Fig. 6. Abnormal projection of proprioceptive afferents in Dcc^–/– mutants. (A,B) 2H3 immunocytochemical staining indicates an absence of 2H3⁺ afferent projections to the ventral horn in E14.5 Dcc^–/– mutant spinal cord (B) when compared with wild-type control (A) (double arrowheads in A,B). 2H3⁺ afferents are aberrantly located in the medial superficial dorsal horn of Dcc^–/– mutant (arrows in B). (C,D) Double immunocytochemical staining of 2H3 and Isl1 in E13.5 wild-type (C) and Dcc^–/– mutant (D) dorsal horns shows that 2H3⁺ afferents (arrows in C,D) appear to follow the pattern of Isl1⁺ staining (arrowheads in C,D). (E,F) DiI labeling of E15.5 wild-type (E) and Dcc^–/– mutant (F) spinal cords shows no proprioceptive afferents growing towards the ventral horn in Dcc^–/– mutant (arrowheads in E,F). Asterisks indicate the heavily labeled superficial dorsal horn by DiI. Scale bars: 100 µm.