Fig. 5. hunchback and Krüppel expression in Of'eve RNAi blastoderms. (A,C,E) Uninjected animals. (B,D1-2,F1-2) Of'eve RNAi animals. (A) Lateral view of uninjected 44-48 hour embryo stained for DNA. Germband has fully invaginated, leaving behind head lobes on outside posterior of yolk ball (densely staining region). Arrow indicates site of invagination. (B) Lateral view of 44-48 hour Of'eve RNAi embryo stained for DNA. Odd wrinkles and putatively mislocalized invagination site (arrow) can be seen. (C) Dorsal aspect of uninjected 36-40 hour blastoderm stained for Oncopeltus hunchback showing that Of'hb normally accumulates in two broad bands. Weaker anterior band corresponds to anterior head, while central band corresponds to posterior mandible through anterior first thoracic segments. (D1) Dorsal view of 36-40 hour Of'eve RNAi blastoderm, showing that Of'hb is now expressed in a single uniform domain in the posterior half of the blastoderm. (D2) Same embryo as in (D1), but rotated to show lateral view. Of'hb expression is restricted to the dorsal part of the blastoderm and is typical in late stage blastoderms. (E) Dorsal view of uninjected 36-40 hour embryo stained for Oncopeltus Krüppel. Of'Kr normally accumulates in posterior one-third of blastoderm. (F1) Of'eve RNAi blastoderm at 36-40 hour. Of'Kr expression is largely absent, with only a very small posterior patch remaining. (F2) Same embryo as in F1, end-on view of posterior tip of blastoderm, showing remaining Of'Kr patch. Scale bars: 200 µm.