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Fig. 5. Oct4 and Nanog are expressed in outer cells of Cdx2-/- blastocysts. (A,B) Fluorescent in situ hybridization of Oct4 (red) and Cdx2 (blue), and YOYO-1 nuclear dye (green). (A) Cdx2+/– or Cdx2+/+ blastocyst. (B) Cdx2-/- blastocyst. Each panel is a composite image of single confocal optical sections. (C,D) Fluorescent whole-mount in situ hybridization of Nanog (red) and YOYO-1 (green). Each panel is a composite projected image of two confocal optical sections. (C) Cdx2+/+ blastocyst. (D) Cdx2-/- blastocyst. Scale bars: 20 µm.