Fig. 7. Effect of DV axial rotation of the hindbrain on the expression of a dorsal otic gene, Gbx2. (A-C) Whole mounts and (D-F) sections of embryos showing expression patterns of Gbx2. Red lines in A-C indicate the level of sections shown in D-F. (A,D) Gbx2 expression in dorsomedial region of control otocyst. (B,C,E,F) Absence of Gbx2 expression in otocysts after DV rotation of the hindbrain (arrowheads). Arrows in C and F indicate Gbx2 expression in the ectopic otocyst that was translocated ventrally during neural tube rotation. D and V in D-F indicate the original dorsal and ventral axes of the neural tube, respectively. Rules in D and E indicate the relative changes in the distance between the dorsal neural tube and otocyst after neural tube rotation. Arrowheads in the insert in F indicate the basement membrane of the ectopic otic epithelial tissue. Scale bars: in A, 100 µm for A-C; in D, 100 µm for D-F.