Fig. 5. Misexpression of Math1 induced ectopic expression of Mbh1 in the spinal cord. Two days after electroporation, mouse embryos were collected at E13.5. Transverse sections at brachial levels of the electroporated spinal cord were hybridized with antisense cRNA probes for Math1 (A), Mbh1 (B) and Lhx9 (C). Right sides of sections were transfected with Math1. Arrowheads indicate endogenous Mbh1⁺ and Lhx9⁺ domains. Ectopic expression of Mbh1 and Lhx9 was also detected at E12.5, one day after electroporation (data not shown), and through all axial levels of the spinal cord at both E12.5 and 13.5 in all electroporated embryos (n=4). Misexpression of EYFP did not induce expression of either Mbh1 or Lhx9 (data not shown). Scale bar: 100 µm.