Fig. 8. Mbh1 function as a transcriptional repressor. Transverse sections at brachial levels of the spinal cord, two days after electroporation at E11.5 of EYFP alone (A), EYFP/Mbh1 (B), EYFP/Math1 (C), EYFP/En-Mbh1 (D), EYFP/VP16-Mbh1 (E) and EYFP/Math1/VP16–Mbh1 (F). Similar patterns of EYFP⁺ axons were observed through all axial levels of the spinal cord in all electroporated EYFP⁺ embryos (n=20, 32, 20, 10, 10 and 10 for EYFP, EYFP/Mbh1, EYFP/Math1, EYFP/En-Mbh1, EYFP/VP16-Mbh1 and EYFP/Math1/VP16–Mbh1, respectively). Arrowheads indicate EYFP⁺ commissural axons. Scale bar: 100 µm.