Fig. 3. Vls is a component of nuage and pole plasm. Fixed, whole-mount egg chambers stained with Oli-Green for DNA and anti-HA antibodies in homozygous vls³ flies expressing P{HA-Vls} (A,C,E,F,H,I,K) and in wild-type flies as controls (B,D,G,J,L). HA-Vls is detected at the posterior pole of the oocyte in transgenic vls³; P{HA-Vls} stage 10 egg chambers (A,C). (C,D) Enlarged views of similar stage 10 oocytes, as shown in A and B, respectively. HA-Vls is observed at the posterior pole of early vls³; P{HA-Vls} embryos and in pole cells (E,F,H,I). (F,G,I,J) Enlarged views of similar syncytial and blastoderm embryos, as shown in E and H, respectively. HA-Vls is detected in the nuage surrounding the nurse cell nuclei (K).