Fig. 5. Displacement of nuage components during early vls oogenesis. (A,B) GFP-Vas and (C,D) Mael accumulate in nuage in stage 7 egg chambers in wild type (A,C) and vls³ (B,D). GFP-Vas (green) was detected in fixed egg chambers stained with Hoechst 33258 for DNA (red). (E,F) Tud accumulates in nuage in wild type stage 7 nurse cells (E), whereas it is homogenously dispersed in the nurse cell cytoplasm in vls³ stage 7 egg chambers (F). Ovaries were stained using anti-Tud antibodies (red) and Oli-Green for DNA (green). Tud is found at high levels in wild type and vls³ oocytes. (G,H) Staining of DNA with Oli-Green reveals that the karyosome, which appears as a single compact dot in wild-type oocytes (G), is frequently fragmented in vls³ oocytes (H). The oocyte nuclear membrane is indicated by a broken line.