Fig. 7. Tud-binding domain in Vls. As performed in Fig. 2C, full-length GST-Vls or derivatives were incubated with S•Tag-Tud9A1-N. Bound S•Tag-Tud9A1-N was detected as described in Fig. 6B. Input: one-tenth of the S•Tag-Tud9A1-N extract. The smallest fragment of Vls showing binding to Tud9A1-N encompasses the first WD domain shown in green. The amount of GST-Vls proteins was visualized by Coomassie staining (lower panel). Representation of the GST-Vls fragments used for mapping and summary of the results are indicated on the right panel.