Fig. 8. (A,B) Paraffin sections of E16.5 stomachs were stained for EAP activity. The bracket indicates corresponding regions of the caudal end of control and mutant stomach. oe, esophagus; Py, pylorus; d, duodenum. (C,D) The junction region of the caudal end of the stomach and the duodenum show slight morphological differences. The gastric parietal cells were stained with anti-H⁺/K⁺-ATPase ß-subunit antibody. (E,F) Anti-GATA4 antibody was used to mark glandular gastric epithelium. (G-J) Paraffin sections of E18.5 stomachs were stained for EAP activity. The bracket indicates corresponding regions of the caudal end of control and mutant stomach. Very low positive signals were found in glandular gastric epithelium of the control (G,I) while highly intense signals were seen in the mutant (H,J). High magnification of the boxed corresponding regions in the control (G) and mutant (H) were shown in I and J, respectively. Scale bar in B: 100 µm for A,B.