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Fig. 6. Developmental expression of AmphiTrk. In all lateral views, the anterior is towards the left and the dorsal is towards the top. (A) Earliest AmphiTrk expression was detected in individual epidermal cells of the ventral midline of the early neurula. (B) The number of expressing cells began to increase at 14 hours of development. (C,D) Left- and right-hand views of the same neurula at 16 hours of development. (E-H) Cross-sections at different levels of the embryos shown in C and D (dotted lines). Positive cells are located within the epidermal layer (arrows) or just beneath it (arrowheads). (I) At 18 hours of development AmphiTrk-expressing cells in ventrolateral positions extend towards dorsal directions. (J) Magnified view of the area inside the rectangle in I, showing the fusiform shaped positive cells and the cytoplasmic distribution of AmphiTrk transcripts. (K) At late neurula stages, dorsalisation of the AmphiTrk signal was more evident. (L-O) In vivo DiI labelling assays. (L) Initial DiI crystal deposition in the ventral epidermis of the hatching neurula (arrows). (M) Cultured late neurula after DiI labelling shown in L. (N) Rhodamine filtered view of M, showing DiI labelled cells (arrows and arrowheads). (O) Merged image of M and N showing labelled cells that migrated individually towards dorsal positions (arrows). (P) In the early larval stage AmphiTrk was upregulated during Hatschek's pit formation (arrow) and weak expression was detected in a rostral patch of cells (arrowhead). (Q) At 26 hours of development, expression was just visible in the developing Hatschek's pit. (R) Magnified view of the area inside the dotted square in T, showing the dorsoposterior expression of AmphiTrk during Hatschek's pit formation. (S) Cross-section at the level of the dotted line in T, showing staining limited to the left-sided dorsolateral part of the Hatschek's pit primordia. (T,U) AmphiTrk expression was maintained in the Hatschek's pit at 28 hours (T) and 30 hours (U) of development. HP, Hatschek's pit; N, notochord; E, endostyle; CV, cerebral vesicle.