Fig. 5. The BOP genes repress the expression of JAG and JGL. Gene expression was analyzed using quantitative RT-PCR. Results were normalized to the expression of 18S ribosomal RNA, then to the value of the wild-type control that was arbitrarily set to 1. Bars represent standard deviation of the mean for three separate biological replicates. (A,C) Relative expression levels of JAG (A) and JGL (C) in leaf 1 and 2 (left) and apical shoots (right) of 11-day-old Col-0 wild-type and bop1-5 bop2-2 mutant plants. (B,D) Relative expression levels of JAG (B) and JGL (D), in leaf 1 and 2 (left) and apical shoots (right) of 8-day-old Col-0 wild-type and bop1-6D mutant plants.