Fig. 5. Nuclear re-organisation of Hoxb in the spinal cord. (A) The distribution of squared interphase distances (d²) in µm² measured between probes for Hoxb1 and Hoxb9, in rhombomeres 5 (r5), spinal cord (SC), and tailbud (Tb) from E9.5 embryos. (B,C) Histogram showing the position of Hoxb9 (B) or Hoxb1 (C) hybridisation signals, relative to the inside, edge or outside of the MMU11 territory, in nuclei from r5 (grey bars), SC (hatched bars) and Tb (black bars) of two E9.5 embryos from different litters. Negative distances indicate signals localised beyond the visible limits of the detectable CT (n 60). (D) Positions (mean±s.e.m.) of Hoxb1 (black squares) and Hoxb9 (white circles) relative to the inside, edge or outside of the MMU11 territory in nuclei located along the anteroposterior axis of the neural tube at E9.5, from r1/r2 down to the tailbud (Tb) (n60).