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Fig. 9. Whole-mount in situ hybridization with Fgf4 (part I), Fgf6 (part II), Fgf8 (part III), Fgfr4 (part IV), scleraxis (parts V,VI) on heterozygous or dKO E10.5 embryos and vibratome sections. (Part I) Fgf4 expression in heterozygous (A,C,E) or dKO (B,D,F) embryos, showing a decrease of Fgf4 somitic expression, AER expression is maintained (A,B). At the interlimb level (C-F), Fgf4 expression is restricted to the central region of the myotome. (Part II) Fgf6 expression in heterozygous (A,C) or dKO (B,D) embryos, showing a severe reduction of Fgf6 in the somites (A,B) of the dKO embryo. Vibratome sections at the interlimb level reveal a strong myotomal expression in the heterozygous embryo (C), while a low signal can be detected in the dKO embryo (D). (Part III) Fgf8 expression in heterozygous (A,D,F) or dKO (B,C,E,G) embryos showing that somitic Fgf8 expression is maintained in the dKO embryos. Fgf8 is expressed in the AER, presomitic mesoderm, otic placode and branchial arches in wild-type or heterozygous embryo (A,D,F). At the somitic level, we detect Fgf8 mRNA, mainly in the caudal lip and more faintly in rostral lip (A,D), allowing Fgf8 to accumulate preferentially in the central myotome (F). In the dKO embryos, presomitic, AER and somitic expression (C,E,G) are preserved, while branchial arches expression (arrows in A and B) is lost (B). (Part IV) Fgfr4 expression in heterozygous (A) or dKO (B) embryos at the interlimb level showing a decrease of Fgfr4 expression in Six1–/–Six4–/– embryos. (Part V) Scleraxis expression in heterozygous (A) or dKO (B) embryos. Scleraxis is expressed in tendon progenitors at the limb level (A), this expression is preserved in the dKO embryo (B), while early somitic syndetomal expression of scleraxis is lost in the dKO embryo (B). (Part VI) Scleraxis is not expressed in Six1 somitic cell population. Double Six1-lacZ (blue) and scleraxis (purple) heterozygous embryo showing the different cell populations of the somites, which express exclusively scleraxis (white arrow) or Six1 (black arrow).