Fig. 1. Expression of Abd-B and cad in the embryonic genital disc. Anterior is to the top. (A-C) Double staining of a late stage 15 hdc-lacZ embryo with anti-ß-galactosidase (green, A, note the three clusters) and anti-Abd-B (red, B) antibodies. A merged image is shown in C. Note that the posterior cluster of genital disc cells is not marked with Abd-B (arrow). (D-F) Double staining of a stage 15 cad-Gal4/UAS-GFP; hdc-lacZ/+ embryo marked with anti-ß-galactosidase (red, D) and GFP (green, E). A merged image is shown in F. See that the posterior cluster (arrow) shows cad expression. (G-L) Embryonic genital disc cells of a UAS-myc-EGFP^F/+; Abd-B-Gal4^LDN/hdc-lacZ embryo, showing expression corresponding to the Abd-B m transcript (GFP expression, green, I-L) in the anterior lateral cells of the disc primordium, marked with an anti-ß-galactosidase antibody (blue, G,J,L). GFP-marked cells are a subset of those expressing Abd-B (red, H,K,L). Note that about four anterior-lateral cells of the disc primordium express GFP (arrows, J) and that some Abd-B-expressing cells are not marked with GFP (arrows, K). A merged image is shown in L. vc, ventral cord.