Fig. 2. Comparative analysis of MTs in living and fixed egg chambers. MTs in
fixed stage-8 egg chambers detected with anti-
-Tubulin-FITC (A)
and anti-Khc (B) (inset in B specificity of the anti-Khc antibody, a
Khc7.288 mutant germline clone shows no anti-Khc staining
in the oocyte). (C-E) Stage 7 egg chambers; (F-H) stage 9 egg
chambers. (C,F) Living egg chambers expressing GFP-
-Tubulin. (D,G)
Fixed egg chambers stained with anti-
-Tubulin-FITC. (E,H) Fixed egg
chambers stained with anti-Khc. (I,J) Stage 9 egg chambers, Khc (red)
and aromatic c-ter amino acid of
Tub (green). The arrow in J points to
the posterior Khc pool.