Fig. 6. grk mutant oocytes conserve the MT configuration of wild-type stage 6 oocytes. (A,B) grk mutant egg chambers stained for anti-Khc. In oocytes comparable to wild-type stage 8 (A) and stage 9 (B), the nucleus is misplaced to the posterior and surrounded by MTs. (C) Schematic representation of the MT network in grk oocytes. (D) grk stage 9 oocyte showing the accumulation of Khc (arrow) between the MT extremities of the long cortical MTs (red arrowheads) and the short central MTs (white arrowhead). (E,F) Wild-type stage 6 oocytes showing a similar MT distribution. MT bundles project from the nucleus at the posterior along the cortex to the anterior, where they bend back to the center (red arrowheads). Short bundles stretch out from the nucleus into the center of the oocyte (white arrowhead). To better visualize MTs, a projection of 10 optical sections (4 µm) is shown in F. (G) Egg chamber expressing Kinesin:ßGal (green) co-stained for Khc (red). (H) Tub37C (R46) localizes to the nucleus at the posterior in grk mutant oocytes and the centrosome close to it (arrow). (I) grk mutant oocyte, showing complete depolymerization of MTs after 30 minutes cold-shock. (J) grk mutant oocyte (projection of 5 µm) after a 15-minute recovery at 25°C. MTs regrow from the misplaced nucleus at the posterior. Asterisk, oocyte nucleus. Scale bar: 20 µm.