Fig. 2. Colonies are derived from a single multipotent renal progenitor. (A) One colony derived from a single cell of EGFP transgenic mesenchyme at culture day 20. (B) RT-PCR of three independent wells containing a single cell-derived colony after 20-day culture. Lanes 1-3: colonies; 4: organ culture of E11.5 mesenchyme rudiments. (C) Lectin staining of 8-week-old adult kidney (left panel) and a single cell-derived colony (right) with PNA (red, podocyte marker) and with LTL (green, proximal tubule marker). (D) Staining of adult kidney (left panel) and a single cell-derived colony (right) with E-cadherin (red, arrow, distal tubule marker) and with LTL (green). Scale bars: 50 µm.