Fig. 5. Colony formation from Sall1-mutant metanephric mesenchyme
obtained from intercrosses of Sall1-GFP mice. (A-F)
Colonies derived from Sall1+/+ (A,B),
Sall1+/- (C,D) and
Sall1-/- (E,F) mesenchyme were stained both
with anti-GFP (A,C,E, green) and with anti-E-cadherin antibodies (B,D,F, red).
Sall1-deficient colonies (E,F) were significantly smaller than those
from wild-type (A,B) and heterozygous (C,D) mesenchyme. Scale bars: 50
µm.