Fig. 7. Effects of reagents on tubulogenesis in organ culture. Whole metanephroi (A-E) and mesenchyme rudiments (F-J) cultured for 7 days. (A,F) Control explants without reagents, (B,G) 10 µmol/l JNK inhibitor 1 (JNKI1), (C,H) 10 µmol/l JNKI2, (D,I) 10 µmol/l Y27,632, (E,J) 20 mmol/l (E) and 10 mmol/l (J) LiCl. (K,L) Section staining using DBA (green), an anti-sFRP2 antibody (red) and DAPI (blue) on whole kidneys treated with 15 µmol/l HIV-TAT peptide (K) and JNKI-1 (L). (M,N) Double labeling with sFRP2 and DAPI on mesenchyme rudiments treated with HIV-TAT peptide (M) and JNKI-1 (N). ub, ureteric bud. Scale bars: 500 µm in A-J; 200 µm in K-N.