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Fig. 4. FGF9 signaling is required for Fgf10 expression. Whole-mount in situ hybridization was performed on E11.5 and E12.5 embryonic GI tracts from wild-type (WT), Fgf9-/- and Fgf10-/- embryos. Fgf10 was expressed in the cecum mesenchyme in WT tissue (A,C), but was completely absent in Fgf9-/- tissue (B,D). By contrast, Fgf9 was expressed in cecum epithelium from WT and Fgf10-/- tissue (E,F). Co, colon; I, terminal ileum.