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Fig. 2. p38 is required for the expression of Nodal (Nod) and Goosecoid (Gsc). (A) Brightfield or DIC images of control (A1), SB-treated (A2), LvNod-overexpressing (A3) and LvGsc-overexpressing (A4) embryos. Doses were 0.2 pg/pl for LvNod and 0.05 pg/pl for LvGsc mRNA. (B) Whole-mount in situ analysis in control (B1,B3) and SB-treated (B2,B4) embryos for LvNod (B1,B2, late gastrula) and LvGsc (B3,B4, prism), shown in vegetal views. (C,D) Whole-mount in situ analysis for Nodal (C) and Gsc (D) in control (top) and SB-treated (bottom) embryos at early blastula (4h, left), late blastula (5.5h, middle) and hatched blastula (7h, right).